Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4288233..4288730 Replicon chromosome
Accession NZ_CP073984
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU92_RS21135 Protein ID WP_001173343.1
Coordinates 4288233..4288406 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU92_RS21140 Protein ID WP_000398619.1
Coordinates 4288434..4288730 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS21125 (4284903) 4284903..4286360 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU92_RS21130 (4286597) 4286597..4288114 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU92_RS21135 (4288233) 4288233..4288406 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU92_RS21140 (4288434) 4288434..4288730 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU92_RS21145 (4288957) 4288957..4289229 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
KFU92_RS21150 (4289241) 4289241..4289471 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
KFU92_RS21155 (4289652) 4289652..4291283 + 1632 WP_121834144.1 sensor histidine kinase -
KFU92_RS21160 (4291280) 4291280..4291999 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200931 WP_001173343.1 NZ_CP073984:c4288406-4288233 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200931 NZ_CP098197:c4548621-4548448 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200931 WP_000398619.1 NZ_CP073984:c4288730-4288434 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200931 NZ_CP098197:c4548945-4548649 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References