Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3533463..3534081 Replicon chromosome
Accession NZ_CP073984
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU92_RS17395 Protein ID WP_001291435.1
Coordinates 3533863..3534081 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU92_RS17390 Protein ID WP_000344800.1
Coordinates 3533463..3533837 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS17380 (3528552) 3528552..3529745 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU92_RS17385 (3529768) 3529768..3532917 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU92_RS17390 (3533463) 3533463..3533837 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU92_RS17395 (3533863) 3533863..3534081 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU92_RS17400 (3534253) 3534253..3534804 + 552 WP_000102568.1 maltose O-acetyltransferase -
KFU92_RS17405 (3534920) 3534920..3535390 + 471 WP_000136192.1 YlaC family protein -
KFU92_RS17410 (3535554) 3535554..3537104 + 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU92_RS17415 (3537146) 3537146..3537499 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU92_RS17425 (3537878) 3537878..3538189 + 312 WP_000409911.1 MGMT family protein -
KFU92_RS17430 (3538220) 3538220..3538792 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200927 WP_001291435.1 NZ_CP073984:3533863-3534081 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200927 NZ_CP098197:4284780-4285121 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200927 WP_000344800.1 NZ_CP073984:3533463-3533837 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200927 NZ_CP098197:c4284785-4284709 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References