Detailed information of TA system TA200914

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1329189..1329814
• Replicon: chromosome
• Accession: NZ_CP073984
• Organism: Escherichia coli strain MB15

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU92_RS06435
• Protein ID: WP_000911330.1
• Coordinates: 1329416..1329814 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU92_RS06430
• Protein ID: WP_000450524.1
• Coordinates: 1329189..1329416 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU92_RS06405 (1324992)	1324992..1325462	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KFU92_RS06410 (1325462)	1325462..1326034	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KFU92_RS06415 (1326180)	1326180..1327058	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU92_RS06420 (1327075)	1327075..1328109	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
KFU92_RS06425 (1328322)	1328322..1329035	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU92_RS06430 (1329189)	1329189..1329416	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU92_RS06435 (1329416)	1329416..1329814	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU92_RS06440 (1329961)	1329961..1330824	+	864	WP_001267508.1	neutral zinc metallopeptidase	-
KFU92_RS06445 (1330839)	1330839..1332854	+	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU92_RS06450 (1332928)	1332928..1333626	+	699	WP_000679823.1	esterase	-
KFU92_RS06455 (1333736)	1333736..1333936	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	algU	1180254..1358008	177754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T200914 WP_000911330.1 NZ_CP073984:1329416-1329814 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T200914 NZ_CP098197:c913272-912895 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGTACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGTCTGATGACCCGCAG
CAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT200914 WP_000450524.1 NZ_CP073984:1329189-1329416 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT200914 NZ_CP098197:c913730-913362 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTAACAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCTGACACTCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33