Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 885897..886551 Replicon chromosome
Accession NZ_CP073984
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name cptA Uniprot ID -
Locus tag KFU92_RS04315 Protein ID WP_032199945.1
Coordinates 886144..886551 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU92_RS04310 Protein ID WP_000354046.1
Coordinates 885897..886163 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS04285 (881185) 881185..881928 + 744 WP_000951964.1 SDR family oxidoreductase -
KFU92_RS04290 (881985) 881985..883418 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KFU92_RS04295 (883463) 883463..883774 + 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
KFU92_RS04300 (883938) 883938..884597 + 660 WP_000250274.1 hemolysin III family protein -
KFU92_RS04305 (884674) 884674..885654 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU92_RS04310 (885897) 885897..886163 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU92_RS04315 (886144) 886144..886551 + 408 WP_032199945.1 protein YgfX Toxin
KFU92_RS04320 (886591) 886591..887112 - 522 WP_001055874.1 flavodoxin FldB -
KFU92_RS04325 (887224) 887224..888120 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU92_RS04330 (888145) 888145..888855 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU92_RS04335 (888861) 888861..890594 + 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16047.04 Da        Isoelectric Point: 11.5204

>T200912 WP_032199945.1 NZ_CP073984:886144-886551 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFNCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200912 NZ_CP098197:264602-264709 [Escherichia coli]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CATGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200912 WP_000354046.1 NZ_CP073984:885897-886163 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200912 NZ_CP098197:c264553-264490 [Escherichia coli]
GTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References