Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/DUF1778(antitoxin)
Location 289821..290621 Replicon chromosome
Accession NZ_CP073984
Organism Escherichia coli strain MB15

Toxin (Protein)


Gene name ataT Uniprot ID F4NNI0
Locus tag KFU92_RS01325 Protein ID WP_000342449.1
Coordinates 290094..290621 (+) Length 176 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID F4NNI1
Locus tag KFU92_RS01320 Protein ID WP_001277108.1
Coordinates 289821..290087 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU92_RS01300 (285479) 285479..286147 + 669 WP_000617723.1 cell division ATP-binding protein FtsE -
KFU92_RS01305 (286140) 286140..287198 + 1059 WP_001042003.1 permease-like cell division protein FtsX -
KFU92_RS01310 (287443) 287443..288297 + 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
KFU92_RS01315 (288568) 288568..289671 + 1104 WP_001021996.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
KFU92_RS01320 (289821) 289821..290087 + 267 WP_001277108.1 type II toxin-antitoxin system antitoxin AtaR Antitoxin
KFU92_RS01325 (290094) 290094..290621 + 528 WP_000342449.1 type II toxin-antitoxin system toxin acetyltransferase AtaT Toxin
KFU92_RS01330 (290618) 290618..291001 - 384 WP_000778795.1 aspartate 1-decarboxylase autocleavage activator PanM -
KFU92_RS01335 (291425) 291425..292534 + 1110 WP_000827696.1 high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK -
KFU92_RS01340 (292582) 292582..293508 + 927 WP_001295111.1 high-affinity branched-chain amino acid ABC transporter permease LivH -
KFU92_RS01345 (293505) 293505..294782 + 1278 WP_000803771.1 branched chain amino acid ABC transporter permease LivM -
KFU92_RS01350 (294779) 294779..295546 + 768 WP_000082101.1 high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(88-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19691.70 Da        Isoelectric Point: 7.7457

>T200910 WP_000342449.1 NZ_CP073984:290094-290621 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNKKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Download         Length: 528 bp

>T200910 NZ_CP098197:263635-263742 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGCATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9910.31 Da        Isoelectric Point: 10.0863

>AT200910 WP_001277108.1 NZ_CP073984:289821-290087 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Download         Length: 267 bp

>AT200910 NZ_CP098197:c263577-263523 [Escherichia coli]
CAAGAATGGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLZ4

Antitoxin

Source ID Structure
PDB 6GTS
PDB 6AJN
PDB 6GTQ
PDB 6GTO
PDB 6GTR
PDB 6AJM
AlphaFold DB A0A829CN24

References