Detailed information of TA system TA200908

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 84083..85706
• Replicon: plasmid pYLMB19a
• Accession: NZ_CP073982
• Organism: Escherichia coli strain MB19

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: H9TH67
• Locus tag: KFU84_RS23540
• Protein ID: WP_000088046.1
• Coordinates: 84381..85706 (+)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: KFU84_RS23535
• Protein ID: WP_024136327.1
• Coordinates: 84083..84376 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU84_RS23505 (KFU84_23320)	79955..81196	+	1242	WP_001053338.1	VWA domain-containing protein	-
KFU84_RS23510	81287..81418	+	132	WP_000374059.1	hypothetical protein	-
KFU84_RS23515 (KFU84_23325)	81759..82040	-	282	WP_000077926.1	hypothetical protein	-
KFU84_RS23520 (KFU84_23330)	82090..82281	-	192	WP_000398480.1	hypothetical protein	-
KFU84_RS23525 (KFU84_23335)	82373..82714	-	342	WP_001151575.1	hypothetical protein	-
KFU84_RS23530 (KFU84_23340)	83087..83479	+	393	WP_000341066.1	hypothetical protein	-
KFU84_RS23535 (KFU84_23345)	84083..84376	+	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
KFU84_RS23540 (KFU84_23350)	84381..85706	+	1326	WP_000088046.1	type II toxin-antitoxin system HipA family toxin	Toxin
KFU84_RS23545 (KFU84_23355)	85767..85973	+	207	WP_000134171.1	hypothetical protein	-
KFU84_RS23550	86075..86212	+	138	WP_233762388.1	hypothetical protein	-
KFU84_RS23555	86224..86466	+	243	WP_233762385.1	hypothetical protein	-
KFU84_RS23560 (KFU84_23365)	86479..87294	+	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
KFU84_RS23565 (KFU84_23370)	87548..87973	+	426	WP_001043843.1	hypothetical protein	-
KFU84_RS23570 (KFU84_23375)	88427..88664	+	238	Protein_86	type I restriction-modification system subunit M N-terminal domain-containing protein	-
KFU84_RS23575 (KFU84_23380)	88719..89018	+	300	WP_001572440.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(A) / tet(A) / blaCTX-M-32	-	1..247187	247187

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50249.20 Da
• Isoelectric Point: 6.1927

>T200908 WP_000088046.1 NZ_CP073982:84381-85706 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEDRWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T200908 NZ_CP098196:82399-82551 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT200908 WP_024136327.1 NZ_CP073982:84083-84376 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT200908 NZ_CP098196:c82349-82287 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A3T0VBU1

Antitoxin

• AlphaFold DB: A0A5Z6V2D1