Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4115477..4116003 Replicon chromosome
Accession NZ_CP073981
Organism Escherichia coli strain MB19

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag KFU84_RS19730 Protein ID WP_000323025.1
Coordinates 4115477..4115764 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag KFU84_RS19735 Protein ID WP_000534858.1
Coordinates 4115764..4116003 (-) Length 80 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU84_RS19695 (4110873) 4110873..4111088 - 216 WP_000839565.1 phage lysis protein EssD -
KFU84_RS19700 (4111340) 4111340..4111714 - 375 WP_001348108.1 cell envelope integrity TolA C-terminal domain-containing protein -
KFU84_RS19705 (4111886) 4111886..4112146 - 261 Protein_3829 cell envelope integrity protein TolA -
KFU84_RS19710 (4113359) 4113359..4113901 - 543 WP_000640161.1 DUF1133 family protein -
KFU84_RS19715 (4113898) 4113898..4114187 - 290 Protein_3831 DUF1364 domain-containing protein -
KFU84_RS19720 (4114187) 4114187..4114786 - 600 WP_130060266.1 DUF1367 family protein -
KFU84_RS19725 (4115250) 4115250..4115405 - 156 WP_096282653.1 type I toxin-antitoxin system toxin HokD -
KFU84_RS19730 (4115477) 4115477..4115764 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
KFU84_RS19735 (4115764) 4115764..4116003 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
KFU84_RS19740 (4116028) 4116028..4116333 + 306 WP_071593935.1 protein YdfV -
KFU84_RS19745 (4116536) 4116536..4116868 + 333 WP_001301033.1 FlxA-like family protein -
KFU84_RS19750 (4117305) 4117305..4117454 - 150 WP_011443592.1 protein YdfW -
KFU84_RS19755 (4117575) 4117575..4118624 - 1050 Protein_3839 ISNCY family transposase -
KFU84_RS19760 (4119526) 4119526..4120005 + 480 WP_250998285.1 MFS transporter -
KFU84_RS19765 (4119899) 4119899..4120642 + 744 WP_251988693.1 MFS transporter -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 4106388..4118645 12257


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.



Sequences


Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T200906 WP_000323025.1 NZ_CP073981:c4115764-4115477 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T200906 NZ_CP098195:c4597338-4597165 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT200906 WP_000534858.1 NZ_CP073981:c4116003-4115764 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT200906 NZ_CP098195:c4597662-4597366 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCTTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CTTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9


Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References