Detailed information of TA system TA200905

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 4037610..4038248
• Replicon: chromosome
• Accession: NZ_CP073981
• Organism: Escherichia coli strain MB19

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU84_RS19355
• Protein ID: WP_000813794.1
• Coordinates: 4038072..4038248 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU84_RS19350
• Protein ID: WP_001270286.1
• Coordinates: 4037610..4038026 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU84_RS19330 (4032762)	4032762..4033703	-	942	WP_001251319.1	ABC transporter permease	-
KFU84_RS19335 (4033704)	4033704..4034717	-	1014	WP_000220393.1	ABC transporter ATP-binding protein	-
KFU84_RS19340 (4034735)	4034735..4035880	-	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
KFU84_RS19345 (4036122)	4036122..4037531	-	1410	WP_000760649.1	PLP-dependent aminotransferase family protein	-
KFU84_RS19350 (4037610)	4037610..4038026	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU84_RS19355 (4038072)	4038072..4038248	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU84_RS19360 (4038470)	4038470..4038700	+	231	WP_000494244.1	YncJ family protein	-
KFU84_RS19365 (4038792)	4038792..4040753	-	1962	WP_032182254.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU84_RS19370 (4040826)	4040826..4041362	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
KFU84_RS19375 (4041415)	4041415..4042644	+	1230	WP_071531711.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4042684..4043832	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T200905 WP_000813794.1 NZ_CP073981:c4038248-4038072 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200905 NZ_CP098195:c4495008-4494658 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200905 WP_001270286.1 NZ_CP073981:c4038026-4037610 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200905 NZ_CP098195:c4495253-4495002 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• PDB: 6HPB