Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpSB/PRK09812-ChpS
Location 1017547..1018142 Replicon chromosome
Accession NZ_CP073981
Organism Escherichia coli strain MB19

Toxin (Protein)


Gene name chpB Uniprot ID U9Y4M4
Locus tag KFU84_RS04990 Protein ID WP_000239579.1
Coordinates 1017547..1017897 (-) Length 117 a.a.

Antitoxin (Protein)


Gene name chpS Uniprot ID U9Y2K1
Locus tag KFU84_RS04995 Protein ID WP_001223208.1
Coordinates 1017891..1018142 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU84_RS04970 (1012995) 1012995..1014017 - 1023 WP_001353963.1 ABC transporter permease -
KFU84_RS04975 (1014031) 1014031..1015533 - 1503 WP_000210561.1 sugar ABC transporter ATP-binding protein -
KFU84_RS04980 (1015674) 1015674..1016630 - 957 WP_000265933.1 galactofuranose ABC transporter substrate-binding protein YtfQ -
KFU84_RS04985 (1016940) 1016940..1017470 + 531 WP_000055072.1 inorganic diphosphatase -
KFU84_RS04990 (1017547) 1017547..1017897 - 351 WP_000239579.1 endoribonuclease toxin ChpB Toxin
KFU84_RS04995 (1017891) 1017891..1018142 - 252 WP_001223208.1 type II toxin-antitoxin system antitoxin ChpS Antitoxin
KFU84_RS05000 (1018354) 1018354..1018695 - 342 WP_001219160.1 gamma-glutamylcyclotransferase -
KFU84_RS05005 (1018698) 1018698..1022477 - 3780 WP_257182184.1 autotransporter assembly complex protein TamB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-114)

Antitoxin

SpoVT-AbrB domain

(6-49)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12511.45 Da        Isoelectric Point: 5.6219

>T200887 WP_000239579.1 NZ_CP073981:c1017897-1017547 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAADEVVEEALLRLQAVVE

Download         Length: 351 bp

>T200887 NZ_CP098195:c98475-98098 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGTTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACTCCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCTGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGAATTC
AGTGCCTGTACCCGTTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCAGGGTAAGTATCCGGAGGCGAAACAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9271.58 Da        Isoelectric Point: 4.4201

>AT200887 WP_001223208.1 NZ_CP073981:c1018142-1017891 [Escherichia coli]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Download         Length: 252 bp

>AT200887 NZ_CP098195:c98896-98522 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGCCCCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGCGCCCGTCTGGTGCAGGAAGGGAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGGGACGCAGATGCATACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGCGGT
GAACTGAATCCCTGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCTTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACTGGCACCCGCAACCACTTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y8A8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LQ26

References