Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3798292..3798946 Replicon chromosome
Accession NZ_CP073974
Organism Escherichia coli strain MB22

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU73_RS18440 Protein ID WP_000244777.1
Coordinates 3798539..3798946 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU73_RS18435 Protein ID WP_000354046.1
Coordinates 3798292..3798558 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU73_RS18410 (3793461) 3793461..3794204 + 744 WP_112919183.1 SDR family oxidoreductase -
KFU73_RS18415 (3794261) 3794261..3795694 - 1434 WP_162721623.1 6-phospho-beta-glucosidase BglA -
KFU73_RS18420 (3795739) 3795739..3796050 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFU73_RS18425 (3796214) 3796214..3796873 + 660 WP_000250274.1 hemolysin III family protein -
KFU73_RS18430 (3797069) 3797069..3798049 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU73_RS18435 (3798292) 3798292..3798558 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU73_RS18440 (3798539) 3798539..3798946 + 408 WP_000244777.1 protein YgfX Toxin
KFU73_RS18445 (3798986) 3798986..3799507 - 522 WP_001055874.1 flavodoxin FldB -
KFU73_RS18450 (3799619) 3799619..3800515 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU73_RS18455 (3800540) 3800540..3801250 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU73_RS18460 (3801256) 3801256..3802989 + 1734 WP_000813200.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200880 WP_000244777.1 NZ_CP073974:3798539-3798946 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200880 NZ_CP073974:3798539-3798946 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200880 WP_000354046.1 NZ_CP073974:3798292-3798558 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200880 NZ_CP073974:3798292-3798558 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References