Detailed information of TA system TA020088

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/-
• Location: 4334293..4334888
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: -
• Locus tag: BN896_3935
• Protein ID: CDJ74454.1
• Coordinates: 4334538..4334888 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: -
• Locus tag: BN896_3934
• Protein ID: CDJ74453.1
• Coordinates: 4334293..4334544 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_3932	4329958..4333737	+	3780	CDJ74451.1	hypothetical protein	-
BN896_3933	4333740..4334081	+	342	CDJ74452.1	hypothetical protein	-
BN896_3934	4334293..4334544	+	252	CDJ74453.1	antitoxin ChpS	Antitoxin
BN896_3935	4334538..4334888	+	351	CDJ74454.1	toxin ChpB	Toxin
BN896_3936	4334968..4335498	-	531	CDJ74455.1	inorganic pyrophosphatase	-
BN896_3937	4335808..4336764	+	957	CDJ74456.1	putative sugar transporter subunit: periplasmic-binding component of ABC superfamily	-
BN896_3938	4336904..4338406	+	1503	CDJ74457.1	putative sugar transporter subunit: ATP-binding component of ABC superfamily	-
BN896_3939	4338417..4339442	+	1026	CDJ74458.1	putative sugar transporter subunit: membrane component of ABC superfamily	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12492.40 Da
• Isoelectric Point: 5.5572

>T20088 CDJ74454.1 HG738867:4334538-4334888 [Escherichia coli str. K-12 substr. MC4100]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLHARLAKRIGLAADEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T20088 HG738867:4334538-4334888 [Escherichia coli str. K-12 substr. MC4100]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACACGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9271.58 Da
• Isoelectric Point: 4.4201

>AT20088 CDJ74453.1 HG738867:4334293-4334544 [Escherichia coli str. K-12 substr. MC4100]
MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT20088 HG738867:4334293-4334544 [Escherichia coli str. K-12 substr. MC4100]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA