Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 2446579..2447076 Replicon chromosome
Accession NZ_CP073974
Organism Escherichia coli strain MB22

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU73_RS11945 Protein ID WP_001173343.1
Coordinates 2446579..2446752 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU73_RS11950 Protein ID WP_000398619.1
Coordinates 2446780..2447076 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU73_RS11935 (2443244) 2443244..2444701 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU73_RS11940 (2444938) 2444938..2446455 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU73_RS11945 (2446579) 2446579..2446752 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU73_RS11950 (2446780) 2446780..2447076 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU73_RS11955 (2447303) 2447303..2447575 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KFU73_RS11960 (2447587) 2447587..2447817 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU73_RS11965 (2447998) 2447998..2449629 + 1632 WP_001216477.1 sensor histidine kinase -
KFU73_RS11970 (2449626) 2449626..2450345 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200877 WP_001173343.1 NZ_CP073974:c2446752-2446579 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200877 NZ_CP098192:2804496-2804603 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200877 WP_000398619.1 NZ_CP073974:c2447076-2446780 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200877 NZ_CP098192:c2804443-2804382 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References