Detailed information of TA system TA200872

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 559101..559627
• Replicon: chromosome
• Accession: NZ_CP073974
• Organism: Escherichia coli strain MB22

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1EXL1
• Locus tag: KFU73_RS02895
• Protein ID: WP_000323025.1
• Coordinates: 559340..559627 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: S1F6D3
• Locus tag: KFU73_RS02890
• Protein ID: WP_000534858.1
• Coordinates: 559101..559340 (+)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU73_RS02860 (554438)	554438..554927	+	490	Protein_560	class I SAM-dependent methyltransferase	-
KFU73_RS02865 (555405)	555405..555824	+	420	Protein_561	hypothetical protein	-
KFU73_RS02870 (556480)	556480..557529	+	1050	Protein_562	ISNCY family transposase	-
KFU73_RS02875 (557650)	557650..557799	+	150	WP_011443592.1	protein YdfW	-
KFU73_RS02880 (558236)	558236..558568	-	333	WP_001301033.1	FlxA-like family protein	-
KFU73_RS02885 (558771)	558771..559076	-	306	WP_071593935.1	protein YdfV	-
KFU73_RS02890 (559101)	559101..559340	+	240	WP_000534858.1	type II toxin-antitoxin system antitoxin RelB	Antitoxin
KFU73_RS02895 (559340)	559340..559627	+	288	WP_000323025.1	type II toxin-antitoxin system mRNA interferase RelE	Toxin
KFU73_RS02900 (559699)	559699..559854	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	-
KFU73_RS02905 (560071)	560071..560322	+	252	WP_001463433.1	protein Rem	-
KFU73_RS02910 (560389)	560389..560667	+	279	WP_001515373.1	hypothetical protein	-
KFU73_RS02915 (560669)	560669..561718	+	1050	WP_001515372.1	DUF968 domain-containing protein	-
KFU73_RS02920 (561736)	561736..562113	+	378	WP_001204787.1	antiterminator Q family protein	-
KFU73_RS02925 (562269)	562269..562793	-	525	WP_000780584.1	lipocalin family protein	-
KFU73_RS02930 (562986)	562986..563945	+	960	WP_000592549.1	DUF523 and DUF1722 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	545614..599914	54300

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-79)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11225.22 Da
• Isoelectric Point: 10.1967

>T200872 WP_000323025.1 NZ_CP073974:559340-559627 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Nucleotide

• Length: 288 bp

>T200872 NZ_CP098192:1987957-1988208 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9071.48 Da
• Isoelectric Point: 4.5230

>AT200872 WP_000534858.1 NZ_CP073974:559101-559340 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Nucleotide

• Length: 240 bp

>AT200872 NZ_CP098192:1988205-1988459 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 4V7K
• PDB: 4V7J
• PDB: 4FXI
• PDB: 4FXE
• PDB: 2KC8
• PDB: 4FXH
• PDB: 2KC9

Antitoxin

• PDB: 4FXE
• PDB: 2KC8
• PDB: 2K29
• AlphaFold DB: A0A4V1CTS8