Detailed information of TA system TA200862

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 65874..66475
• Replicon: plasmid pYLMB31b
• Accession: NZ_CP073964
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: doc
• Uniprot ID: U9YA20
• Locus tag: KFU79_RS24455
• Protein ID: WP_001216045.1
• Coordinates: 65874..66254 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: KFU79_RS24460
• Protein ID: WP_001190712.1
• Coordinates: 66254..66475 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU79_RS24430 (KFU79_24205)	61314..62798	-	1485	WP_000124155.1	hypothetical protein	-
KFU79_RS24435 (KFU79_24210)	62798..63991	-	1194	WP_000219605.1	terminase	-
KFU79_RS24440 (KFU79_24215)	64078..64530	-	453	WP_001369802.1	hypothetical protein	-
KFU79_RS24445 (KFU79_24220)	64619..65662	-	1044	WP_000648823.1	DUF968 domain-containing protein	-
KFU79_RS24450 (KFU79_24225)	65690..65869	-	180	WP_000113019.1	hypothetical protein	-
KFU79_RS24455 (KFU79_24230)	65874..66254	-	381	WP_001216045.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KFU79_RS24460 (KFU79_24235)	66254..66475	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU79_RS24465 (KFU79_24240)	66548..66937	-	390	WP_000506726.1	S24 family peptidase	-
KFU79_RS24470 (KFU79_24245)	66998..68218	-	1221	WP_000343760.1	ISL3-like element ISKox3 family transposase	-
KFU79_RS24475 (KFU79_24250)	68385..68636	-	252	WP_001377386.1	DNA polymerase III subunit theta	-
KFU79_RS24480 (KFU79_24255)	69086..69223	+	138	WP_000123562.1	hypothetical protein	-
KFU79_RS24485 (KFU79_24260)	69298..69660	-	363	WP_001261544.1	hypothetical protein	-
KFU79_RS24490 (KFU79_24265)	69657..70589	-	933	WP_077780118.1	hypothetical protein	-
KFU79_RS24495 (KFU79_24270)	70571..70945	-	375	WP_023154376.1	hypothetical protein	-
KFU79_RS24500 (KFU79_24275)	70952..71245	-	294	WP_001677496.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..98314	98314
	-	flank	IS/Tn	-	-	66998..68218	1220

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (3-120)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13588.29 Da
• Isoelectric Point: 5.1514

>T200862 WP_001216045.1 NZ_CP073964:c66254-65874 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T200862 NZ_CP098192:310398-310628 [Escherichia coli]
CTGGATCAGCTATTTAAATCACCTTTACCTCAAGGAATCAAATGGACTGATATAGAATCTCTGGTTAAAGCGTTAGGCGG
AGAAATTAAGGAAGGTAGGGGGTCGCGCTGTAAGTTCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGT
CGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTGCGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT200862 WP_001190712.1 NZ_CP073964:c66475-66254 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT200862 NZ_CP098192:310625-310984 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Structures

Toxin

• AlphaFold DB: A0A829CLP7

Antitoxin

• AlphaFold DB: A0A829CJB6