Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 111109..111752 Replicon plasmid pYLMB31a
Accession NZ_CP073963
Organism Escherichia coli strain MB31

Toxin (Protein)


Gene name vagD Uniprot ID V0UN72
Locus tag KFU79_RS24035 Protein ID WP_001034044.1
Coordinates 111336..111752 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID B1P7N7
Locus tag KFU79_RS24030 Protein ID WP_001261286.1
Coordinates 111109..111339 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU79_RS24010 (107740) 107740..108495 - 756 WP_000852146.1 replication initiation protein RepE -
KFU79_RS24015 (109217) 109217..110023 - 807 WP_000016970.1 site-specific integrase -
KFU79_RS24020 (110024) 110024..110329 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB -
KFU79_RS24025 (110331) 110331..110549 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA -
KFU79_RS24030 (111109) 111109..111339 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
KFU79_RS24035 (111336) 111336..111752 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin Toxin
KFU79_RS24040 (111827) 111827..113392 + 1566 WP_001128474.1 AAA family ATPase -
KFU79_RS24045 (113377) 113377..114399 + 1023 WP_000361402.1 helicase UvrD -
KFU79_RS24050 (114653) 114653..115339 - 687 Protein_148 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aac(3)-IIa / blaTEM-1B / sitABCD iutA / iucD / iucC / iucB / iucA 1..133598 133598
- flank IS/Tn - - 114653..115000 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15232.66 Da        Isoelectric Point: 6.8536

>T200861 WP_001034044.1 NZ_CP073963:111336-111752 [Escherichia coli]
VNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRA
AVDATTEIKVALRLAGTPIGPNDTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR

Download         Length: 417 bp

>T200861 NZ_CP098191:c62280-62128 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8747.86 Da        Isoelectric Point: 4.3775

>AT200861 WP_001261286.1 NZ_CP073963:111109-111339 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFLELEKADPDFMTEREDVVSDEGRVNL

Download         Length: 231 bp

>AT200861 NZ_CP098191:62332-62392 [Escherichia coli]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CHW1

Antitoxin

Source ID Structure
AlphaFold DB A0A829CKZ6

References