Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 110024..110549 Replicon plasmid pYLMB31a
Accession NZ_CP073963
Organism Escherichia coli strain MB31

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag KFU79_RS24020 Protein ID WP_001159871.1
Coordinates 110024..110329 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID H9TJP1
Locus tag KFU79_RS24025 Protein ID WP_000813630.1
Coordinates 110331..110549 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU79_RS24005 (105986) 105986..107152 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
KFU79_RS24010 (107740) 107740..108495 - 756 WP_000852146.1 replication initiation protein RepE -
KFU79_RS24015 (109217) 109217..110023 - 807 WP_000016970.1 site-specific integrase -
KFU79_RS24020 (110024) 110024..110329 - 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
KFU79_RS24025 (110331) 110331..110549 - 219 WP_000813630.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
KFU79_RS24030 (111109) 111109..111339 + 231 WP_001261286.1 type II toxin-antitoxin system VapB family antitoxin -
KFU79_RS24035 (111336) 111336..111752 + 417 WP_001034044.1 type II toxin-antitoxin system VapC family toxin -
KFU79_RS24040 (111827) 111827..113392 + 1566 WP_001128474.1 AAA family ATPase -
KFU79_RS24045 (113377) 113377..114399 + 1023 WP_000361402.1 helicase UvrD -
KFU79_RS24050 (114653) 114653..115339 - 687 Protein_148 IS1 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid tet(B) / aac(3)-IIa / blaTEM-1B / sitABCD iutA / iucD / iucC / iucB / iucA 1..133598 133598
- flank IS/Tn - - 114653..115000 347


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T200860 WP_001159871.1 NZ_CP073963:c110329-110024 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T200860 NZ_CP098191:c15816-15538 [Escherichia coli]
ATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGATGGC
CGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTCGTG
TGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTGTAT
ATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8354.27 Da        Isoelectric Point: 4.5203

>AT200860 WP_000813630.1 NZ_CP073963:c110549-110331 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT200860 NZ_CP098191:c16085-15816 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
CGGCAGGACGTCAGCAACTCGGGTGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CEF5

References