Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4373732..4374229 Replicon chromosome
Accession NZ_CP073962
Organism Escherichia coli strain MB31

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU79_RS21135 Protein ID WP_001173343.1
Coordinates 4373732..4373905 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU79_RS21140 Protein ID WP_000398619.1
Coordinates 4373933..4374229 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU79_RS21125 (4370402) 4370402..4371859 + 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -
KFU79_RS21130 (4372096) 4372096..4373613 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU79_RS21135 (4373732) 4373732..4373905 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU79_RS21140 (4373933) 4373933..4374229 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU79_RS21145 (4374456) 4374456..4374728 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KFU79_RS21150 (4374740) 4374740..4374970 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU79_RS21155 (4375151) 4375151..4376782 + 1632 WP_001216477.1 sensor histidine kinase -
KFU79_RS21160 (4376779) 4376779..4377498 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200852 WP_001173343.1 NZ_CP073962:c4373905-4373732 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200852 NZ_CP098187:c2915188-2915086 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200852 WP_000398619.1 NZ_CP073962:c4374229-4373933 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200852 NZ_CP098187:2915048-2915316 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References