Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4156126..4156702 Replicon chromosome
Accession NZ_CP073962
Organism Escherichia coli strain MB31

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag KFU79_RS20005 Protein ID WP_001297643.1
Coordinates 4156415..4156702 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag KFU79_RS20000 Protein ID WP_000063160.1
Coordinates 4156126..4156428 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU79_RS19985 (4152722) 4152722..4154872 + 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
KFU79_RS19990 (4154922) 4154922..4155125 + 204 WP_000467859.1 YbdD/YjiX family protein -
KFU79_RS19995 (4155136) 4155136..4156092 + 957 WP_001297640.1 GTPase -
KFU79_RS20000 (4156126) 4156126..4156428 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
KFU79_RS20005 (4156415) 4156415..4156702 - 288 WP_001297643.1 BrnT family toxin Toxin
KFU79_RS20010 (4156901) 4156901..4157597 - 697 Protein_3911 IS1 family transposase -
KFU79_RS20015 (4157912) 4157912..4159144 + 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
KFU79_RS20020 (4159185) 4159185..4160465 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4156901..4157110 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T200850 WP_001297643.1 NZ_CP073962:c4156702-4156415 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T200850 NZ_CP098187:c2324065-2323958 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT200850 WP_000063160.1 NZ_CP073962:c4156428-4156126 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT200850 NZ_CP098187:2324118-2324179 [Escherichia coli]
TGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References