Detailed information of TA system TA200845

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2895699..2896543
• Replicon: chromosome
• Accession: NZ_CP073962
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B1LJY4
• Locus tag: KFU79_RS14110
• Protein ID: WP_000854686.1
• Coordinates: 2895699..2896082 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0J1S3
• Locus tag: KFU79_RS14115
• Protein ID: WP_001285602.1
• Coordinates: 2896163..2896543 (-)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU79_RS14070 (2890702)	2890702..2891193	-	492	WP_001300785.1	DUF1097 domain-containing protein	-
KFU79_RS14075 (2891295)	2891295..2891849	-	555	WP_001001921.1	molecular chaperone YcdY	-
KFU79_RS14080 (2891873)	2891873..2892610	-	738	WP_000283667.1	zinc-binding phosphatase	-
KFU79_RS14085 (2892665)	2892665..2893603	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KFU79_RS14095 (2894074)	2894074..2894915	-	842	Protein_2759	DUF4942 domain-containing protein	-
KFU79_RS14100 (2895000)	2895000..2895197	-	198	WP_000839253.1	DUF957 domain-containing protein	-
KFU79_RS14105 (2895214)	2895214..2895702	-	489	WP_032180032.1	DUF5983 family protein	-
KFU79_RS14110 (2895699)	2895699..2896082	-	384	WP_000854686.1	TA system toxin CbtA family protein	Toxin
KFU79_RS14115 (2896163)	2896163..2896543	-	381	WP_001285602.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU79_RS14120 (2896554)	2896554..2897237	-	684	WP_000086768.1	hypothetical protein	-
KFU79_RS14125 (2897256)	2897256..2897477	-	222	WP_000692298.1	DUF987 domain-containing protein	-
KFU79_RS14130 (2897540)	2897540..2898016	-	477	WP_001186726.1	RadC family protein	-
KFU79_RS14135 (2898032)	2898032..2898517	-	486	WP_000214307.1	antirestriction protein	-
KFU79_RS14140 (2898609)	2898609..2899430	-	822	WP_001761104.1	DUF932 domain-containing protein	-
KFU79_RS14145 (2899531)	2899531..2899739	-	209	Protein_2769	DUF905 domain-containing protein	-
KFU79_RS14150 (2899840)	2899840..2900295	-	456	WP_000581506.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-113)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14272.28 Da
• Isoelectric Point: 6.8614

>T200845 WP_000854686.1 NZ_CP073962:c2896082-2895699 [Escherichia coli]
MKTLPDTHVRAASRCPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYVLVRTDQPGF
SAGAPSSQLINSIDILRARRATGLMTRDNYRMVNNITLGKHPEEAKQ

Nucleotide

• Length: 384 bp

>T200845 NZ_CP098187:c831459-831121 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCAATTAACCGTGAGTTA
TGCGAGTCGCTACCCGGATTACAACCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CCGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAAGAGAGCGAA
CTGATGCAGTCACTGCGCCAGGTGTGCAAGCTGTCGGCGCGTAAACAAAAGCAGGTGCAGGATTTTATTGGGGTGATTAG
TAACAAAACGCCACGCTAA

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13938.69 Da
• Isoelectric Point: 5.0823

>AT200845 WP_001285602.1 NZ_CP073962:c2896543-2896163 [Escherichia coli]
VSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT200845 NZ_CP098187:831454-831530 [Escherichia coli]
AGTCATAACTGCTATTCCCTATAAATAGTGATTGTGATGAGCGGTGCGGGTGTGTTGGCGCACATCCGCACCGCGCT