Detailed information of TA system TA200844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2466297..2466935
• Replicon: chromosome
• Accession: NZ_CP073962
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0A6TXU8
• Locus tag: KFU79_RS11855
• Protein ID: WP_001447010.1
• Coordinates: 2466759..2466935 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU79_RS11850
• Protein ID: WP_001270286.1
• Coordinates: 2466297..2466713 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU79_RS11830 (2461449)	2461449..2462390	-	942	WP_001251304.1	ABC transporter permease	-
KFU79_RS11835 (2462391)	2462391..2463404	-	1014	WP_257171187.1	ABC transporter ATP-binding protein	-
KFU79_RS11840 (2463422)	2463422..2464567	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KFU79_RS11845 (2464812)	2464812..2466218	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KFU79_RS11850 (2466297)	2466297..2466713	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU79_RS11855 (2466759)	2466759..2466935	-	177	WP_001447010.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU79_RS11860 (2467157)	2467157..2467387	+	231	WP_000494244.1	YncJ family protein	-
KFU79_RS11865 (2467479)	2467479..2469440	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU79_RS11870 (2469513)	2469513..2470049	-	537	WP_000429155.1	DNA-binding transcriptional regulator SutR	-
KFU79_RS11875 (2470141)	2470141..2471316	+	1176	WP_001236265.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6779.86 Da
• Isoelectric Point: 11.2298

>T200844 WP_001447010.1 NZ_CP073962:c2466935-2466759 [Escherichia coli]
VKQSEFRRWLESQGVDVANSSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200844 NZ_CP098187:513159-513332 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTTCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200844 WP_001270286.1 NZ_CP073962:c2466713-2466297 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200844 NZ_CP098187:512835-513131 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAACTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAGCATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A0A6TXU8