Detailed information of TA system TA020084

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/-
• Location: 3872442..3873025
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: BN896_2517
• Protein ID: CDJ74047.1
• Coordinates: 3872690..3873025 (+)
• Length: 112 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: BN896_2518
• Protein ID: CDJ74046.1
• Coordinates: 3872442..3872690 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_2523	3867445..3868746	+	1302	CDJ74042.1	23S rRNA 5-methyluridine methyltransferase	-
BN896_2521	3869145..3869510	+	366	CDJ74043.1	insertion sequence 2 OrfA protein	-
BN896_2520	3869468..3870373	+	906	CDJ74044.1	insertion element IS2 transposase InsD	-
BN896_2519	3870358..3872364	+	2007	CDJ74045.1	GTP diphosphokinase	-
BN896_2518	3872442..3872690	+	249	CDJ74046.1	antitoxin MazE	Antitoxin
BN896_2517	3872690..3873025	+	336	CDJ74047.1	toxin ChpA	Toxin
BN896_2516	3873096..3873887	+	792	CDJ74048.1	nucleoside triphosphate pyrophosphohydrolase	-
BN896_2515	3874115..3875752	+	1638	CDJ74049.1	CTP synthetase	-
BN896_2514	3875840..3877138	+	1299	CDJ74050.1	phosphopyruvate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 112 a.a.
• Molecular weight: 12098.04 Da
• Isoelectric Point: 8.2618

>T20084 CDJ74047.1 HG738867:3872690-3873025 [Escherichia coli str. K-12 substr. MC4100]
MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Nucleotide

• Length: 336 bp

>T20084 HG738867:3872690-3873025 [Escherichia coli str. K-12 substr. MC4100]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9355.74 Da
• Isoelectric Point: 4.4665

>AT20084 CDJ74046.1 HG738867:3872442-3872690 [Escherichia coli str. K-12 substr. MC4100]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Nucleotide

• Length: 249 bp

>AT20084 HG738867:3872442-3872690 [Escherichia coli str. K-12 substr. MC4100]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA