Detailed information of TA system TA200830

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 802775..803610
• Replicon: chromosome
• Accession: NZ_CP073962
• Organism: Escherichia coli strain MB31

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A376WVB1
• Locus tag: KFU79_RS03900
• Protein ID: WP_001094448.1
• Coordinates: 802775..803152 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KFU79_RS03905
• Protein ID: WP_074417293.1
• Coordinates: 803242..803610 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU79_RS03870 (798183)	798183..799160	+	978	WP_000633209.1	type II secretion system minor pseudopilin GspK	-
KFU79_RS03875 (799157)	799157..800335	+	1179	WP_000094991.1	type II secretion system protein GspL	-
KFU79_RS03880 (800337)	800337..800873	+	537	WP_000942785.1	GspM family type II secretion system protein YghD	-
KFU79_RS03885 (801154)	801154..801996	-	843	WP_021532534.1	DUF4942 domain-containing protein	-
KFU79_RS03890 (802081)	802081..802278	-	198	WP_000839243.1	DUF957 domain-containing protein	-
KFU79_RS03895 (802290)	802290..802778	-	489	WP_000761714.1	DUF5983 family protein	-
KFU79_RS03900 (802775)	802775..803152	-	378	WP_001094448.1	TA system toxin CbtA family protein	Toxin
KFU79_RS03905 (803242)	803242..803610	-	369	WP_074417293.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KFU79_RS03910 (803773)	803773..803994	-	222	WP_000692345.1	DUF987 domain-containing protein	-
KFU79_RS03915 (804057)	804057..804533	-	477	WP_001186727.1	RadC family protein	-
KFU79_RS03920 (804549)	804549..805034	-	486	WP_032286264.1	antirestriction protein	-
KFU79_RS03925 (805089)	805089..805907	-	819	WP_257171222.1	DUF932 domain-containing protein	-
KFU79_RS03930 (806007)	806007..806240	-	234	WP_257171223.1	DUF905 family protein	-
KFU79_RS03935 (806319)	806319..806774	-	456	WP_257171224.1	IrmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	801027..862723	61696

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14221.22 Da
• Isoelectric Point: 8.2919

>T200830 WP_001094448.1 NZ_CP073962:c803152-802775 [Escherichia coli]
MNTLPDTHVREASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T200830 NZ_CP098183:2250009-2250185 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13538.40 Da
• Isoelectric Point: 5.9562

>AT200830 WP_074417293.1 NZ_CP073962:c803610-803242 [Escherichia coli]
VSDALSGTMLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT200830 NZ_CP098183:2250231-2250647 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A376WVB1