Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4275859..4276356 Replicon chromosome
Accession NZ_CP073958
Organism Escherichia coli strain MB46

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag KFU88_RS21110 Protein ID WP_001173343.1
Coordinates 4275859..4276032 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag KFU88_RS21115 Protein ID WP_000398619.1
Coordinates 4276060..4276356 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU88_RS21100 (4272529) 4272529..4273986 + 1458 WP_000856841.1 dipeptide/tripeptide permease DtpC -
KFU88_RS21105 (4274223) 4274223..4275740 + 1518 WP_001295074.1 lysine--tRNA ligase -
KFU88_RS21110 (4275859) 4275859..4276032 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
KFU88_RS21115 (4276060) 4276060..4276356 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
KFU88_RS21120 (4276583) 4276583..4276855 - 273 WP_000405651.1 GNAT family N-acetyltransferase -
KFU88_RS21125 (4276867) 4276867..4277097 - 231 WP_000371704.1 4Fe-4S mono-cluster protein YjdI -
KFU88_RS21130 (4277278) 4277278..4278909 + 1632 WP_001216477.1 sensor histidine kinase -
KFU88_RS21135 (4278906) 4278906..4279625 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T200825 WP_001173343.1 NZ_CP073958:c4276032-4275859 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T200825 NZ_CP098183:c1971291-1971184 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT200825 WP_000398619.1 NZ_CP073958:c4276356-4276060 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT200825 NZ_CP098183:1971339-1971405 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTAAACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References