Detailed information of TA system TA200824

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4264075..4264910
• Replicon: chromosome
• Accession: NZ_CP073958
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KFU88_RS21065
• Protein ID: WP_000854821.1
• Coordinates: 4264533..4264910 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: KFU88_RS21060
• Protein ID: WP_001285610.1
• Coordinates: 4264075..4264443 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU88_RS21025 (4259877)	4259877..4260761	+	885	WP_023563932.1	50S ribosome-binding GTPase	-
KFU88_RS21030 (4260880)	4260880..4261557	+	678	WP_063091580.1	hypothetical protein	-
KFU88_RS21035 (4261563)	4261563..4261715	+	153	WP_001696589.1	DUF905 family protein	-
KFU88_RS21040 (4261816)	4261816..4262634	+	819	WP_171935502.1	DUF932 domain-containing protein	-
KFU88_RS21045 (4262716)	4262716..4263195	+	480	WP_000860074.1	antirestriction protein	-
KFU88_RS21050 (4263211)	4263211..4263687	+	477	WP_063091579.1	RadC family protein	-
KFU88_RS21055 (4263774)	4263774..4263995	+	222	WP_000692350.1	DUF987 domain-containing protein	-
KFU88_RS21060 (4264075)	4264075..4264443	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU88_RS21065 (4264533)	4264533..4264910	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KFU88_RS21070 (4264907)	4264907..4265104	+	198	Protein_4118	DUF5983 family protein	-
KFU88_RS21075 (4265132)	4265132..4265329	+	198	WP_023563519.1	DUF957 domain-containing protein	-
KFU88_RS21080 (4265414)	4265414..4266256	+	843	WP_001280501.1	DUF4942 domain-containing protein	-
KFU88_RS21085 (4267005)	4267005..4268543	+	1539	WP_001751945.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T200824 WP_000854821.1 NZ_CP073958:4264533-4264910 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T200824 NZ_CP098183:c1158673-1158455 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT200824 WP_001285610.1 NZ_CP073958:4264075-4264443 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT200824 NZ_CP098183:c1159073-1158699 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7W4PV54