Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3533631..3534249 Replicon chromosome
Accession NZ_CP073958
Organism Escherichia coli strain MB46

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU88_RS17490 Protein ID WP_001291435.1
Coordinates 3534031..3534249 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU88_RS17485 Protein ID WP_000344800.1
Coordinates 3533631..3534005 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU88_RS17475 (3528720) 3528720..3529913 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU88_RS17480 (3529936) 3529936..3533085 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU88_RS17485 (3533631) 3533631..3534005 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU88_RS17490 (3534031) 3534031..3534249 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU88_RS17495 (3534421) 3534421..3534972 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU88_RS17500 (3535088) 3535088..3535558 + 471 WP_000136192.1 YlaC family protein -
KFU88_RS17505 (3535722) 3535722..3537272 + 1551 WP_032265872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU88_RS17510 (3537314) 3537314..3537667 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU88_RS17520 (3538046) 3538046..3538357 + 312 WP_000409911.1 MGMT family protein -
KFU88_RS17525 (3538388) 3538388..3538960 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200821 WP_001291435.1 NZ_CP073958:3534031-3534249 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200821 NZ_CP098183:524579-524929 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200821 WP_000344800.1 NZ_CP073958:3533631-3534005 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200821 NZ_CP098183:524334-524585 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References