Detailed information of TA system TA200820

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2870732..2871566
• Replicon: chromosome
• Accession: NZ_CP073958
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: W0S379
• Locus tag: KFU88_RS14310
• Protein ID: WP_000854808.1
• Coordinates: 2870732..2871109 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: KFU88_RS14315
• Protein ID: WP_085313956.1
• Coordinates: 2871198..2871566 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU88_RS14270 (2865739)	2865739..2866230	-	492	WP_001300785.1	DUF1097 domain-containing protein	-
KFU88_RS14275 (2866332)	2866332..2866886	-	555	WP_001001921.1	molecular chaperone YcdY	-
KFU88_RS14280 (2866910)	2866910..2867647	-	738	WP_000283667.1	zinc-binding phosphatase	-
KFU88_RS14285 (2867702)	2867702..2868640	-	939	WP_000351317.1	glyoxylate/hydroxypyruvate reductase GhrA	-
KFU88_RS14295 (2869111)	2869111..2869953	-	843	WP_063101836.1	DUF4942 domain-containing protein	-
KFU88_RS14300 (2870038)	2870038..2870235	-	198	WP_029396577.1	DUF957 domain-containing protein	-
KFU88_RS14305 (2870247)	2870247..2870735	-	489	WP_257187874.1	DUF5983 family protein	-
KFU88_RS14310 (2870732)	2870732..2871109	-	378	WP_000854808.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KFU88_RS14315 (2871198)	2871198..2871566	-	369	WP_085313956.1	type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA	Antitoxin
KFU88_RS14320 (2871616)	2871616..2872260	-	645	WP_257187875.1	antitoxin of toxin-antitoxin stability system	-
KFU88_RS14325 (2872275)	2872275..2872496	-	222	WP_000692312.1	DUF987 domain-containing protein	-
KFU88_RS14330 (2872565)	2872565..2873041	-	477	WP_001186742.1	RadC family protein	-
KFU88_RS14335 (2873056)	2873056..2873541	-	486	WP_063101663.1	antirestriction protein	-
KFU88_RS14340 (2873632)	2873632..2874450	-	819	WP_096200128.1	DUF932 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	csgB / csgD / csgE / csgF / csgG	2862120..2916822	54702

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13909.93 Da
• Isoelectric Point: 7.9085

>T200820 WP_000854808.1 NZ_CP073958:c2871109-2870732 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T200820 NZ_CP098183:428714-428887 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13650.41 Da
• Isoelectric Point: 6.4757

>AT200820 WP_085313956.1 NZ_CP073958:c2871566-2871198 [Escherichia coli]
VSDTLSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNQLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYARGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT200820 NZ_CP098183:428390-428686 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: W0S379