Detailed information of TA system TA200819

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2507772..2508410
• Replicon: chromosome
• Accession: NZ_CP073958
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: KFU88_RS12445
• Protein ID: WP_000813794.1
• Coordinates: 2508234..2508410 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU88_RS12440
• Protein ID: WP_001270286.1
• Coordinates: 2507772..2508188 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU88_RS12420 (2502924)	2502924..2503865	-	942	WP_001251313.1	ABC transporter permease	-
KFU88_RS12425 (2503866)	2503866..2504879	-	1014	WP_000220399.1	ABC transporter ATP-binding protein	-
KFU88_RS12430 (2504897)	2504897..2506042	-	1146	WP_000047424.1	ABC transporter substrate-binding protein	-
KFU88_RS12435 (2506287)	2506287..2507693	-	1407	WP_000760626.1	PLP-dependent aminotransferase family protein	-
KFU88_RS12440 (2507772)	2507772..2508188	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU88_RS12445 (2508234)	2508234..2508410	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU88_RS12450 (2508632)	2508632..2508862	+	231	WP_000494244.1	YncJ family protein	-
KFU88_RS12455 (2508954)	2508954..2510915	-	1962	WP_001752477.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU88_RS12460 (2510988)	2510988..2511524	-	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
KFU88_RS12465 (2511577)	2511577..2512806	+	1230	WP_096218838.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2512846..2513994	1148

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T200819 WP_000813794.1 NZ_CP073958:c2508410-2508234 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200819 NZ_CP098183:156886-157197 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200819 WP_001270286.1 NZ_CP073958:c2508188-2507772 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200819 NZ_CP098183:157198-157488 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• PDB: 6HPB