Detailed information of TA system TA200807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1326927..1327552
• Replicon: chromosome
• Accession: NZ_CP073958
• Organism: Escherichia coli strain MB46

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU88_RS06525
• Protein ID: WP_000911330.1
• Coordinates: 1327154..1327552 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: KFU88_RS06520
• Protein ID: WP_000450524.1
• Coordinates: 1326927..1327154 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU88_RS06495 (1322729)	1322729..1323199	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
KFU88_RS06500 (1323199)	1323199..1323771	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
KFU88_RS06505 (1323917)	1323917..1324795	+	879	WP_001311023.1	4-hydroxy-tetrahydrodipicolinate synthase	-
KFU88_RS06510 (1324812)	1324812..1325846	+	1035	WP_257187958.1	outer membrane protein assembly factor BamC	-
KFU88_RS06515 (1326059)	1326059..1326772	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
KFU88_RS06520 (1326927)	1326927..1327154	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU88_RS06525 (1327154)	1327154..1327552	+	399	WP_000911330.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU88_RS06530 (1327699)	1327699..1328562	+	864	WP_001267498.1	neutral zinc metallopeptidase	-
KFU88_RS06535 (1328577)	1328577..1330592	+	2016	WP_000829336.1	tRNA cytosine(34) acetyltransferase TmcA	-
KFU88_RS06540 (1330666)	1330666..1331364	+	699	WP_000679812.1	esterase	-
KFU88_RS06545 (1331474)	1331474..1331674	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T200807 WP_000911330.1 NZ_CP073958:1327154-1327552 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T200807 NZ_CP098178:c2977967-2977716 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT200807 WP_000450524.1 NZ_CP073958:1326927-1327154 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT200807 NZ_CP098178:c2977719-2977465 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33