Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 904407..905061 Replicon chromosome
Accession NZ_CP073958
Organism Escherichia coli strain MB46

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU88_RS04560 Protein ID WP_000244777.1
Coordinates 904654..905061 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU88_RS04555 Protein ID WP_000354046.1
Coordinates 904407..904673 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU88_RS04535 (900376) 900376..901809 - 1434 WP_213838684.1 6-phospho-beta-glucosidase BglA -
KFU88_RS04540 (901854) 901854..902165 + 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
KFU88_RS04545 (902329) 902329..902988 + 660 WP_000250294.1 hemolysin III family protein -
KFU88_RS04550 (903184) 903184..904164 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU88_RS04555 (904407) 904407..904673 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU88_RS04560 (904654) 904654..905061 + 408 WP_000244777.1 protein YgfX Toxin
KFU88_RS04565 (905101) 905101..905622 - 522 WP_001055874.1 flavodoxin FldB -
KFU88_RS04570 (905734) 905734..906630 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU88_RS04575 (906655) 906655..907365 + 711 WP_001753033.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU88_RS04580 (907371) 907371..909104 + 1734 WP_000813188.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200805 WP_000244777.1 NZ_CP073958:904654-905061 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200805 NZ_CP098178:c2805047-2804944 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200805 WP_000354046.1 NZ_CP073958:904407-904673 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200805 NZ_CP098178:2804906-2805177 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References