Detailed information of TA system TA200799

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 3472..3994
• Replicon: plasmid pYLMB49c
• Accession: NZ_CP073956
• Organism: Escherichia coli strain MB49

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A9LK42
• Locus tag: KFU90_RS24255
• Protein ID: WP_000220562.1
• Coordinates: 3713..3994 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: KFU90_RS24250
• Protein ID: WP_000121743.1
• Coordinates: 3472..3723 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU90_RS24230 (KFU90_24070)	423..779	-	357	WP_000876433.1	DNA distortion polypeptide 3	-
KFU90_RS24235 (KFU90_24075)	916..1362	-	447	WP_001074382.1	hypothetical protein	-
KFU90_RS24240 (KFU90_24080)	1402..2238	-	837	WP_001802123.1	replication initiation protein	-
KFU90_RS24245	3249..3380	-	132	WP_001378418.1	hypothetical protein	-
KFU90_RS24250 (KFU90_24085)	3472..3723	+	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
KFU90_RS24255 (KFU90_24090)	3713..3994	+	282	WP_000220562.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU90_RS24260 (KFU90_24095)	4140..4463	+	324	WP_001181905.1	hypothetical protein	-
KFU90_RS24265 (KFU90_24100)	4511..4756	+	246	WP_000356542.1	hypothetical protein	-
KFU90_RS24270 (KFU90_24105)	4746..4961	+	216	WP_257188516.1	hypothetical protein	-
KFU90_RS24275 (KFU90_24110)	5054..5383	+	330	WP_000866654.1	hypothetical protein	-
KFU90_RS24280 (KFU90_24115)	5426..5605	+	180	WP_000439079.1	hypothetical protein	-
KFU90_RS24285 (KFU90_24120)	5675..5824	+	150	WP_000003879.1	hypothetical protein	-
KFU90_RS24290 (KFU90_24125)	5837..6109	+	273	WP_000160399.1	hypothetical protein	-
KFU90_RS24295 (KFU90_24135)	6435..6980	+	546	WP_020801480.1	DNA distortion polypeptide 1	-
KFU90_RS24300 (KFU90_24140)	6983..8149	+	1167	WP_105394073.1	MobP1 family relaxase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38404	38404

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11021.83 Da
• Isoelectric Point: 10.5938

>T200799 WP_000220562.1 NZ_CP073956:3713-3994 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVISVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T200799 NZ_CP098178:c1253468-1253250 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT200799 WP_000121743.1 NZ_CP073956:3472-3723 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT200799 NZ_CP098178:c1253868-1253494 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A9LK42

Antitoxin

• AlphaFold DB: A0A3S5I301