Detailed information of TA system TA200795

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 80395..81020
• Replicon: plasmid pYLMB49a
• Accession: NZ_CP073954
• Organism: Escherichia coli strain MB49

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KFU90_RS23665
• Protein ID: WP_000911308.1
• Coordinates: 80395..80793 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8S7CUH2
• Locus tag: KFU90_RS23670
• Protein ID: WP_000450522.1
• Coordinates: 80793..81020 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU90_RS23645 (77348)	77348..77956	+	609	Protein_98	hypothetical protein	-
KFU90_RS23650 (78388)	78388..79050	+	663	WP_235412092.1	complement resistance protein TraT	-
KFU90_RS23655 (79178)	79178..79870	+	693	WP_000235172.1	hypothetical protein	-
KFU90_RS23660 (80012)	80012..80380	+	369	WP_235412088.1	DUF2726 domain-containing protein	-
KFU90_RS23665 (80395)	80395..80793	-	399	WP_000911308.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU90_RS23670 (80793)	80793..81020	-	228	WP_000450522.1	toxin-antitoxin system antitoxin VapB	Antitoxin
KFU90_RS23675 (81153)	81153..83339	+	2187	WP_149517080.1	type IV conjugative transfer system coupling protein TraD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / aph(3'')-Ib / aph(6)-Id	-	1..94480	94480

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14972.34 Da
• Isoelectric Point: 8.1432

>T200795 WP_000911308.1 NZ_CP073954:c80793-80395 [Escherichia coli]
MLKFMLDTNICIFTIKNKPAHIRERFNLNTGRMCISSVTVMELVYGAEKSQFPERNLAIIEGFISRLDVLDYDCAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERIAGLRLEDWC

Nucleotide

• Length: 399 bp

>T200795 NZ_CP098178:157776-158087 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8368.36 Da
• Isoelectric Point: 4.3569

>AT200795 WP_000450522.1 NZ_CP073954:c81020-80793 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVDVIAVGRTRIITPAGESWDSWFDGDSVSADFMNDREQPAVQERDAF

Nucleotide

• Length: 228 bp

>AT200795 NZ_CP098178:158088-158378 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG