Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3481434..3482052 Replicon chromosome
Accession NZ_CP073953
Organism Escherichia coli strain MB49

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU90_RS16790 Protein ID WP_001291435.1
Coordinates 3481834..3482052 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU90_RS16785 Protein ID WP_000344800.1
Coordinates 3481434..3481808 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU90_RS16775 (3476523) 3476523..3477716 + 1194 WP_128995125.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU90_RS16780 (3477739) 3477739..3480888 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU90_RS16785 (3481434) 3481434..3481808 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU90_RS16790 (3481834) 3481834..3482052 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU90_RS16795 (3482223) 3482223..3482774 + 552 WP_000102578.1 maltose O-acetyltransferase -
KFU90_RS16800 (3482890) 3482890..3483360 + 471 WP_000136192.1 YlaC family protein -
KFU90_RS16805 (3483524) 3483524..3485074 + 1551 WP_001365886.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU90_RS16810 (3485116) 3485116..3485469 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU90_RS16820 (3485848) 3485848..3486159 + 312 WP_000409911.1 MGMT family protein -
KFU90_RS16825 (3486190) 3486190..3486762 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200784 WP_001291435.1 NZ_CP073953:3481834-3482052 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200784 NZ_CP098175:44928-45311 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200784 WP_000344800.1 NZ_CP073953:3481434-3481808 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200784 NZ_CP098175:44686-44928 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References