Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 864387..865041 Replicon chromosome
Accession NZ_CP073953
Organism Escherichia coli strain MB49

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag KFU90_RS04240 Protein ID WP_000244777.1
Coordinates 864634..865041 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag KFU90_RS04235 Protein ID WP_000354046.1
Coordinates 864387..864653 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU90_RS04210 (859556) 859556..860299 + 744 WP_000951948.1 SDR family oxidoreductase -
KFU90_RS04215 (860356) 860356..861789 - 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
KFU90_RS04220 (861834) 861834..862145 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
KFU90_RS04225 (862309) 862309..862968 + 660 WP_000250274.1 hemolysin III family protein -
KFU90_RS04230 (863164) 863164..864144 - 981 WP_000886062.1 tRNA-modifying protein YgfZ -
KFU90_RS04235 (864387) 864387..864653 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
KFU90_RS04240 (864634) 864634..865041 + 408 WP_000244777.1 protein YgfX Toxin
KFU90_RS04245 (865081) 865081..865602 - 522 WP_001055870.1 flavodoxin FldB -
KFU90_RS04250 (865714) 865714..866610 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
KFU90_RS04255 (866635) 866635..867345 + 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
KFU90_RS04260 (867351) 867351..869084 + 1734 WP_128995306.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T200771 WP_000244777.1 NZ_CP073953:864634-865041 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T200771 NZ_CP098169:c1325761-1325477 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCAGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT200771 WP_000354046.1 NZ_CP073953:864387-864653 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT200771 NZ_CP098169:c1325993-1325751 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References