Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 45841..46442 Replicon plasmid pYLMB50a
Accession NZ_CP073950
Organism Escherichia coli strain MB50

Toxin (Protein)


Gene name doc Uniprot ID U9YA20
Locus tag KFU75_RS22865 Protein ID WP_001216045.1
Coordinates 45841..46221 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag KFU75_RS22870 Protein ID WP_001190712.1
Coordinates 46221..46442 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU75_RS22835 (KFU75_22660) 40857..41330 - 474 WP_004201280.1 trimethoprim-resistant dihydrofolate reductase DfrA14 -
KFU75_RS22840 (KFU75_22665) 41486..42499 + 1014 WP_000845048.1 class 1 integron integrase IntI1 -
KFU75_RS22845 (KFU75_22670) 42934..43638 - 705 WP_001067855.1 IS6-like element IS26 family transposase -
KFU75_RS22850 (KFU75_22675) 43704..45218 + 1515 Protein_46 Tn3-like element TnAs1 family transposase -
KFU75_RS22855 (KFU75_22680) 45252..45629 - 378 Protein_47 hypothetical protein -
KFU75_RS22860 (KFU75_22685) 45657..45836 - 180 WP_000113019.1 hypothetical protein -
KFU75_RS22865 (KFU75_22690) 45841..46221 - 381 WP_001216045.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
KFU75_RS22870 (KFU75_22695) 46221..46442 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
KFU75_RS22875 (KFU75_22700) 46625..48181 + 1557 WP_016231378.1 type I restriction-modification system subunit M -
KFU75_RS22880 (KFU75_22705) 48178..49413 + 1236 WP_059338147.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid blaTEM-1B / blaLAP-2 / qnrS1 / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 - 1..117974 117974


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(3-120)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13588.29 Da        Isoelectric Point: 5.1514

>T200767 WP_001216045.1 NZ_CP073950:c46221-45841 [Escherichia coli]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T200767 NZ_CP098167:c5311106-5310723 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT200767 WP_001190712.1 NZ_CP073950:c46442-46221 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT200767 NZ_CP098167:c5311348-5311106 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CLP7

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References