Detailed information of TA system TA200765

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4089227..4089749
• Replicon: chromosome
• Accession: NZ_CP073949
• Organism: Escherichia coli strain MB50

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: KFU75_RS19895
• Protein ID: WP_072651623.1
• Coordinates: 4089227..4089517 (-)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: V0SC69
• Locus tag: KFU75_RS19900
• Protein ID: WP_000212715.1
• Coordinates: 4089507..4089749 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU75_RS19880 (4084440)	4084440..4086095	+	1656	WP_072651625.1	alpha,alpha-phosphotrehalase	-
KFU75_RS19885 (4086489)	4086489..4088627	+	2139	WP_000187805.1	anaerobic ribonucleoside-triphosphate reductase	-
KFU75_RS19890 (4088762)	4088762..4089226	+	465	WP_001106232.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
KFU75_RS19895 (4089227)	4089227..4089517	-	291	WP_072651623.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFU75_RS19900 (4089507)	4089507..4089749	-	243	WP_000212715.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KFU75_RS19905 (4089940)	4089940..4090326	-	387	WP_001232246.1	cytochrome b562	-
KFU75_RS19910 (4090379)	4090379..4091731	-	1353	WP_001162192.1	metalloprotease PmbA	-
KFU75_RS19915 (4091825)	4091825..4092376	+	552	WP_000166277.1	ribosome biogenesis factor YjgA	-
KFU75_RS19920 (4092532)	4092532..4093905	-	1374	WP_001219783.1	UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-82)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-72)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11308.25 Da
• Isoelectric Point: 10.1701

>T200765 WP_072651623.1 NZ_CP073949:c4089517-4089227 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLGDVLKRPRNPSAKLLDLPDCYKIKLRTLGYRRVYQVNDKELLVLVIAIGK
RENSAVYEDVTKRLDE

Nucleotide

• Length: 291 bp

>T200765 NZ_CP098167:c4826585-4826301 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9125.56 Da
• Isoelectric Point: 5.1369

>AT200765 WP_000212715.1 NZ_CP073949:c4089749-4089507 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Nucleotide

• Length: 243 bp

>AT200765 NZ_CP098167:c4826817-4826575 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Structures

Antitoxin

• AlphaFold DB: A0A7U9H4B3