Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3447756..3448374 Replicon chromosome
Accession NZ_CP073949
Organism Escherichia coli strain MB50

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU75_RS16875 Protein ID WP_001291435.1
Coordinates 3448156..3448374 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU75_RS16870 Protein ID WP_000344800.1
Coordinates 3447756..3448130 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU75_RS16860 (3442845) 3442845..3444038 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU75_RS16865 (3444061) 3444061..3447210 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU75_RS16870 (3447756) 3447756..3448130 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU75_RS16875 (3448156) 3448156..3448374 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU75_RS16880 (3448546) 3448546..3449097 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU75_RS16885 (3449213) 3449213..3449683 + 471 WP_000136192.1 YlaC family protein -
KFU75_RS16890 (3449847) 3449847..3451397 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU75_RS16895 (3451439) 3451439..3451792 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU75_RS16905 (3452171) 3452171..3452482 + 312 WP_000409911.1 MGMT family protein -
KFU75_RS16910 (3452513) 3452513..3453085 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200762 WP_001291435.1 NZ_CP073949:3448156-3448374 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200762 NZ_CP098167:4061606-4061824 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200762 WP_000344800.1 NZ_CP073949:3447756-3448130 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200762 NZ_CP098167:4061205-4061579 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References