Detailed information of TA system TA020076

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/-
• Location: 1966065..1966896
• Replicon: chromosome
• Accession: HG738867
• Organism: Escherichia coli str. K-12 substr. MC4100

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: BN896_1796
• Protein ID: CDJ72376.1
• Coordinates: 1966522..1966896 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: BN896_1795
• Protein ID: CDJ72375.1
• Coordinates: 1966065..1966433 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BN896_1792	1963732..1965264	+	1533	CDJ72372.1	CP4-44 prophage; putative membrane protein	-
BN896_1793	1965261..1965707	+	447	CDJ72373.1	CP4-44 prophage; putative DNA repair protein	-
BN896_1794	1965770..1965991	+	222	CDJ72374.1	CP4-44 prophage protein	-
BN896_1795	1966065..1966433	+	369	CDJ72375.1	CP4-44 prophage; antitoxin of the YeeV-YeeU toxin-antitoxin system	Antitoxin
BN896_1796	1966522..1966896	+	375	CDJ72376.1	CP4-44 prophage; toxin of the YeeV-YeeU toxin-antitoxin system	Toxin
BN896_1797	1967985..1968314	-	330	CDJ72377.1	hypothetical protein	-
BN896_1798	1968486..1969544	-	1059	CDJ72378.1	hypothetical protein	-
BN896_1799	1969742..1970215	-	474	CDJ72379.1	DNA gyrase inhibitor	-
BN896_1800	1970334..1971500	-	1167	CDJ72380.1	D-alanyl-D-alanine carboxypeptidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T20076 CDJ72376.1 HG738867:1966522-1966896 [Escherichia coli str. K-12 substr. MC4100]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T20076 HG738867:1966522-1966896 [Escherichia coli str. K-12 substr. MC4100]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACACTGAATGACACACCTTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13651.52 Da
• Isoelectric Point: 5.9541

>AT20076 CDJ72375.1 HG738867:1966065-1966433 [Escherichia coli str. K-12 substr. MC4100]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCDYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT20076 HG738867:1966065-1966433 [Escherichia coli str. K-12 substr. MC4100]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGATTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA