Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 1784193..1785024 Replicon chromosome
Accession NZ_CP073949
Organism Escherichia coli strain MB50

Toxin (Protein)


Gene name yeeV Uniprot ID S1P968
Locus tag KFU75_RS08580 Protein ID WP_000854814.1
Coordinates 1784193..1784567 (-) Length 125 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID B3Y195
Locus tag KFU75_RS08585 Protein ID WP_001285585.1
Coordinates 1784656..1785024 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU75_RS08540 (1779589) 1779589..1780755 + 1167 WP_257187374.1 serine-type D-Ala-D-Ala carboxypeptidase DacD -
KFU75_RS08545 (1780874) 1780874..1781347 + 474 WP_001105415.1 DNA gyrase inhibitor SbmC -
KFU75_RS08550 (1781545) 1781545..1782603 + 1059 WP_001200891.1 FUSC family protein -
KFU75_RS08555 (1782775) 1782775..1783104 + 330 WP_000450409.1 DUF496 family protein -
KFU75_RS08560 (1783205) 1783205..1783339 - 135 WP_257187408.1 EutP/PduV family microcompartment system protein -
KFU75_RS08565 (1783459) 1783459..1783587 + 129 Protein_1673 transposase domain-containing protein -
KFU75_RS08570 (1783876) 1783876..1783956 - 81 Protein_1674 hypothetical protein -
KFU75_RS08575 (1784002) 1784002..1784196 - 195 WP_000988600.1 DUF5983 family protein -
KFU75_RS08580 (1784193) 1784193..1784567 - 375 WP_000854814.1 type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA Toxin
KFU75_RS08585 (1784656) 1784656..1785024 - 369 WP_001285585.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
KFU75_RS08590 (1785098) 1785098..1785319 - 222 WP_000692323.1 DUF987 domain-containing protein -
KFU75_RS08595 (1785382) 1785382..1785858 - 477 WP_001186773.1 RadC family protein -
KFU75_RS08600 (1785874) 1785874..1786353 - 480 WP_000860076.1 antirestriction protein -
KFU75_RS08605 (1786435) 1786435..1787256 - 822 WP_137548930.1 DUF932 domain-containing protein -
KFU75_RS08610 (1787495) 1787495..1787887 - 393 WP_066018936.1 hypothetical protein -
KFU75_RS08615 (1787903) 1787903..1788586 - 684 WP_000775500.1 hypothetical protein -
KFU75_RS08620 (1788722) 1788722..1789792 - 1071 WP_000102643.1 patatin-like phospholipase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 125 a.a.        Molecular weight: 13898.94 Da        Isoelectric Point: 7.8522

>T200751 WP_000854814.1 NZ_CP073949:c1784567-1784193 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Download         Length: 375 bp

>T200751 NZ_CP098165:c3343086-3342984 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13593.48 Da        Isoelectric Point: 6.3139

>AT200751 WP_001285585.1 NZ_CP073949:c1785024-1784656 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Download         Length: 369 bp

>AT200751 NZ_CP098165:3342977-3343122 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FY50

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LW60

References