Detailed information of TA system TA200743

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4323029..4323526
• Replicon: chromosome
• Accession: NZ_CP073946
• Organism: Escherichia coli strain MB51

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: L4JJG6
• Locus tag: KFU68_RS21245
• Protein ID: WP_001173343.1
• Coordinates: 4323029..4323202 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: -
• Locus tag: KFU68_RS21250
• Protein ID: WP_072280288.1
• Coordinates: 4323230..4323526 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU68_RS21235 (4319694)	4319694..4321151	+	1458	WP_000856829.1	dipeptide/tripeptide permease DtpC	-
KFU68_RS21240 (4321388)	4321388..4322905	+	1518	WP_001295074.1	lysine--tRNA ligase	-
KFU68_RS21245 (4323029)	4323029..4323202	-	174	WP_001173343.1	type V toxin-antitoxin system toxin GhoT	Toxin
KFU68_RS21250 (4323230)	4323230..4323526	-	297	WP_072280288.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
KFU68_RS21255 (4323753)	4323753..4324025	-	273	WP_202792988.1	GNAT family N-acetyltransferase	-
KFU68_RS21260 (4324037)	4324037..4324267	-	231	WP_000371700.1	4Fe-4S mono-cluster protein YjdI	-
KFU68_RS21265 (4324448)	4324448..4326079	+	1632	WP_001216477.1	sensor histidine kinase	-
KFU68_RS21270 (4326076)	4326076..4326795	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6555.06 Da
• Isoelectric Point: 10.2288

>T200743 WP_001173343.1 NZ_CP073946:c4323202-4323029 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Nucleotide

• Length: 174 bp

>T200743 NZ_CP098165:44933-45316 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11482.00 Da
• Isoelectric Point: 4.1705

>AT200743 WP_072280288.1 NZ_CP073946:c4323526-4323230 [Escherichia coli]
MEGKNKFNTYIVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT200743 NZ_CP098165:44691-44933 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Structures

Toxin

• AlphaFold DB: A0A7U9QI41