Detailed information of TA system TA200741

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4274004..4274602
• Replicon: chromosome
• Accession: NZ_CP073946
• Organism: Escherichia coli strain MB51

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A8T6PRV3
• Locus tag: KFU68_RS20985
• Protein ID: WP_053291281.1
• Coordinates: 4274004..4274381 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: L4J1C0
• Locus tag: KFU68_RS20990
• Protein ID: WP_001603498.1
• Coordinates: 4274381..4274602 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU68_RS20965 (4270656)	4270656..4271396	-	741	WP_000755102.1	outer membrane beta-barrel protein	-
KFU68_RS20970 (4272341)	4272341..4273411	+	1071	WP_112026432.1	ESPR domain-containing protein	-
KFU68_RS20975 (4273408)	4273408..4273611	+	204	WP_143357953.1	hypothetical protein	-
KFU68_RS20980 (4273679)	4273679..4273894	+	216	Protein_4102	transposase	-
KFU68_RS20985 (4274004)	4274004..4274381	-	378	WP_053291281.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
KFU68_RS20990 (4274381)	4274381..4274602	-	222	WP_001603498.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFU68_RS20995 (4274787)	4274787..4275806	-	1020	WP_257201870.1	fimbrial protein	-
KFU68_RS21000 (4275818)	4275818..4278304	-	2487	WP_063101695.1	fimbrial biogenesis outer membrane usher protein	-
KFU68_RS21005 (4278322)	4278322..4279002	-	681	WP_053270475.1	fimbrial assembly chaperone	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	htpB	4250823..4310360	59537
	-	flank	IS/Tn	-	-	4273679..4273960	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (4-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13487.28 Da
• Isoelectric Point: 6.2257

>T200741 WP_053291281.1 NZ_CP073946:c4274381-4274004 [Escherichia coli]
MRHISPEELIAIHDANISRYGGLPGMPDSGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVYDSPELAELTVGAATGEVSVSTVVATLRRLYGTA

Nucleotide

• Length: 378 bp

>T200741 NZ_CP098161:c4998108-4997740 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8190.24 Da
• Isoelectric Point: 6.3159

>AT200741 WP_001603498.1 NZ_CP073946:c4274602-4274381 [Escherichia coli]
MQSINFRTARSNLTEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDTTNKKLVNR

Nucleotide

• Length: 222 bp

>AT200741 NZ_CP098161:c4998326-4998105 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Antitoxin

• AlphaFold DB: L4J1C0