Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3562756..3563374 Replicon chromosome
Accession NZ_CP073946
Organism Escherichia coli strain MB51

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag KFU68_RS17440 Protein ID WP_001291435.1
Coordinates 3563156..3563374 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag KFU68_RS17435 Protein ID WP_000344800.1
Coordinates 3562756..3563130 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU68_RS17425 (3557845) 3557845..3559038 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFU68_RS17430 (3559061) 3559061..3562210 + 3150 WP_001132469.1 efflux RND transporter permease AcrB -
KFU68_RS17435 (3562756) 3562756..3563130 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
KFU68_RS17440 (3563156) 3563156..3563374 + 219 WP_001291435.1 HHA domain-containing protein Toxin
KFU68_RS17445 (3563546) 3563546..3564097 + 552 WP_000102564.1 maltose O-acetyltransferase -
KFU68_RS17450 (3564213) 3564213..3564683 + 471 WP_000136192.1 YlaC family protein -
KFU68_RS17455 (3564847) 3564847..3566397 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
KFU68_RS17460 (3566439) 3566439..3566792 - 354 WP_000878140.1 DUF1428 domain-containing protein -
KFU68_RS17470 (3567171) 3567171..3567482 + 312 WP_000409911.1 MGMT family protein -
KFU68_RS17475 (3567513) 3567513..3568085 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T200737 WP_001291435.1 NZ_CP073946:3563156-3563374 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T200737 NZ_CP098161:c2821043-2820726 [Klebsiella pneumoniae]
ATGTTGACTTTTATTGAACTACAAGGTTTCAGTAAGCGACGCCAGGTGCTGTTGCAGGATGATGAATTTCGGGCCTTTCA
GGAAATCCTTATAAACGATCCCGAGGCTGGGGACACAATTTCGGGAACCGGTGGTTTTCGTAAAATTCGCTGGAGTCGTC
CCGGGATGGGGAAGCGCGGCGGTGTACGAGTGATTTACTATAATGTAACGAAAAAGGGAAGAATTTACCTGGCGCTCATC
TATCCTAAAAATGAACAGGATGAGTTAAATCAGGAGCAAAAAAAGATGTTGAGACAAGTGGCTGATTTAATAAACTAG

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT200737 WP_000344800.1 NZ_CP073946:3562756-3563130 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT200737 NZ_CP098161:c2820650-2820354 [Klebsiella pneumoniae]
ATGAAAGACGAATTGTTTGCCGATCTGCTGGCGAGTGCAGAAGAAATGGTCAGGATCGAGAAAGGTGAACAAATGCTGAT
GGAGCAGCACGTTCATACCTTCAGCGAAATTGACGTGAAAGCGATACGTGAAGCCACAGGTTTAAAACAGCAGGACTTTG
CAGCGGCCGTTGGCGTTAGCTACGAGTTGGTTAAAAGCTGGGAAACGAAACGACGTCACCCAACCGGTGCAGCCAGAAAG
CTGCTTTTATTGCTTCAGGGTAATCCATTGATTATCAACTTGTTGAAGGTTGTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References