Detailed information of TA system TA200717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 50843..51486
• Replicon: plasmid pYLMB52a
• Accession: NZ_CP073944
• Organism: Escherichia coli strain MB52

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q84A06
• Locus tag: KFU89_RS22250
• Protein ID: WP_000754566.1
• Coordinates: 50843..51259 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: KFU89_RS22255
• Protein ID: WP_001261276.1
• Coordinates: 51256..51486 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU89_RS22230 (KFU89_22090)	45947..48022	+	2076	WP_000205526.1	protease Lon-related BREX system protein BrxL	-
KFU89_RS22235 (KFU89_22095)	48130..48408	-	279	Protein_41	IS3 family transposase	-
KFU89_RS22240 (KFU89_22100)	48425..49501	-	1077	WP_000227969.1	IS110 family transposase	-
KFU89_RS22245 (KFU89_22105)	49783..50639	-	857	Protein_43	IS3-like element ISEc15 family transposase	-
KFU89_RS22250 (KFU89_22110)	50843..51259	-	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	Toxin
KFU89_RS22255 (KFU89_22115)	51256..51486	-	231	WP_001261276.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
KFU89_RS22260 (KFU89_22120)	52060..52410	+	351	WP_000493378.1	hypothetical protein	-
KFU89_RS22265 (KFU89_22125)	52461..53204	+	744	WP_000129823.1	hypothetical protein	-
KFU89_RS22270 (KFU89_22130)	53201..53977	+	777	WP_000015958.1	site-specific integrase	-
KFU89_RS22275 (KFU89_22135)	54035..54292	-	258	WP_000764642.1	hypothetical protein	-
KFU89_RS22280 (KFU89_22140)	54421..54525	-	105	WP_032409716.1	hypothetical protein	-
KFU89_RS22285 (KFU89_22145)	55055..55921	+	867	WP_004118283.1	replication initiation protein	-
KFU89_RS22290 (KFU89_22150)	56098..56367	-	270	WP_000339857.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	tet(A) / qnrS1 / dfrA14 / aph(6)-Id / aph(3'')-Ib	-	1..67961	67961

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15006.52 Da
• Isoelectric Point: 9.2957

>T200717 WP_000754566.1 NZ_CP073944:c51259-50843 [Escherichia coli]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T200717 NZ_CP098159:357834-358202 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT200717 WP_001261276.1 NZ_CP073944:c51486-51256 [Escherichia coli]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT200717 NZ_CP098159:357616-357837 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A387K1G3

Antitoxin

• AlphaFold DB: A0A387K023