Detailed information of TA system TA200713

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 3593092..3593771
• Replicon: chromosome
• Accession: NZ_CP073943
• Organism: Escherichia coli strain MB52

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: S1FHS4
• Locus tag: KFU89_RS17285
• Protein ID: WP_000854680.1
• Coordinates: 3593430..3593771 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: -
• Locus tag: KFU89_RS17280
• Protein ID: WP_089606835.1
• Coordinates: 3593092..3593409 (+)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU89_RS17235 (3588270)	3588270..3588971	+	702	WP_006812588.1	WYL domain-containing protein	-
KFU89_RS17240 (3589012)	3589012..3589248	+	237	WP_001144031.1	protein YpjK	-
KFU89_RS17245 (3589248)	3589248..3589691	+	444	WP_000824223.1	lipoprotein YafY	-
KFU89_RS17250 (3589714)	3589714..3590181	+	468	WP_001547765.1	protein YkfB	-
KFU89_RS17255 (3590329)	3590329..3591462	+	1134	WP_248792371.1	integrase core domain-containing protein	-
KFU89_RS17260 (3591556)	3591556..3591795	+	240	WP_000194654.1	DUF905 family protein	-
KFU89_RS17265 (3591893)	3591893..3592351	+	459	WP_000211838.1	antirestriction protein	-
KFU89_RS17270 (3592367)	3592367..3592843	+	477	WP_000811693.1	RadC family protein	-
KFU89_RS17275 (3592852)	3592852..3593073	+	222	WP_000691994.1	DUF987 domain-containing protein	-
KFU89_RS17280 (3593092)	3593092..3593409	+	318	WP_089606835.1	type IV toxin-antitoxin system antitoxin YafW	Antitoxin
KFU89_RS17285 (3593430)	3593430..3593771	+	342	WP_000854680.1	type IV toxin-antitoxin system toxin YkfI	Toxin
KFU89_RS17295 (3594343)	3594343..3595596	-	1254	WP_000893279.1	glutamate-5-semialdehyde dehydrogenase	-
KFU89_RS17300 (3595608)	3595608..3596711	-	1104	WP_001285288.1	glutamate 5-kinase	-
KFU89_RS17305 (3596999)	3596999..3598054	+	1056	WP_000749863.1	phosphoporin PhoE	-
KFU89_RS17310 (3598093)	3598093..3598494	-	402	WP_000174677.1	sigma factor-binding protein Crl	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12892.95 Da
• Isoelectric Point: 9.6543

>T200713 WP_000854680.1 NZ_CP073943:3593430-3593771 [Escherichia coli]
MKTLPATTQRAVKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
SWQEQSPYLRAADILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T200713 NZ_CP098149:c4703037-4702696 [Klebsiella pneumoniae]
ATGAAAACTCTACCTGCAACAACTCCGCAGGCGGTGACGCTATGTCTGTCGTCTGTGGCAGTGTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACACTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCCGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAAGGGATTC
AACTGGCAAGAACACTCCCCATATCTGCGGGCAGTTGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGCGCCGAAG
CCGTAAAAATGCTGTACGTTAA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11958.64 Da
• Isoelectric Point: 7.7496

>AT200713 WP_089606835.1 NZ_CP073943:3593092-3593409 [Escherichia coli]
MSNPTRGLQREITLRLGARLVQEGNRLHYLADRASITGKFSDIECRKLDKTFPHFIRQMESMLTTGELSPNHAHCVTLYH
NDLTCEADTLGSCGYVYIAIYPTQR

Nucleotide

• Length: 318 bp

>AT200713 NZ_CP098149:c4703399-4703058 [Klebsiella pneumoniae]
ATGAAATCTACTGACTCAGAGAACGATTCATTTCTCAAGTGGGGTCTGAATCGTAATGTGACACCTTGCTTTGGCGCTCG
TCTGGTACAGGAGGGAAACCGACTTCACTATCTGGCTGACCGGGCCAGCATTACCGGTGAATTCAGCAACGCAGAATCTT
TGAAGTTGGATGAGGTATTCCCACATTTTATCAGCCAGATGGAGTCGATGCTGACTACTGGTGAAATGAATCCTCGCCAT
GCCCACTTTTTCACCCTGTACCACAATGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTACGGCTACGTATACATCGC
CGTTTACCCCACTCAACGTTAA

Structures

Toxin

• AlphaFold DB: S1FHS4