Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2389607..2390245 Replicon chromosome
Accession NZ_CP073943
Organism Escherichia coli strain MB52

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag KFU89_RS11430 Protein ID WP_000813794.1
Coordinates 2390069..2390245 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag KFU89_RS11425 Protein ID WP_001270286.1
Coordinates 2389607..2390023 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU89_RS11405 (2384759) 2384759..2385700 - 942 WP_001251313.1 ABC transporter permease -
KFU89_RS11410 (2385701) 2385701..2386714 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
KFU89_RS11415 (2386732) 2386732..2387877 - 1146 WP_000047424.1 ABC transporter substrate-binding protein -
KFU89_RS11420 (2388122) 2388122..2389528 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
KFU89_RS11425 (2389607) 2389607..2390023 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
KFU89_RS11430 (2390069) 2390069..2390245 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
KFU89_RS11435 (2390467) 2390467..2390697 + 231 WP_000494244.1 YncJ family protein -
KFU89_RS11440 (2390789) 2390789..2392750 - 1962 WP_001752477.1 23S rRNA 5-hydroxycytidine C2501 synthase -
KFU89_RS11445 (2392823) 2392823..2393359 - 537 WP_000429141.1 DNA-binding transcriptional regulator SutR -
KFU89_RS11450 (2393451) 2393451..2394641 + 1191 WP_001236269.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2394681..2395829 1148


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T200711 WP_000813794.1 NZ_CP073943:c2390245-2390069 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T200711 NZ_CP098149:4030725-4030943 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT200711 WP_001270286.1 NZ_CP073943:c2390023-2389607 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT200711 NZ_CP098149:4030324-4030698 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References