Detailed information of TA system TA200695

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 718494..719329
• Replicon: chromosome
• Accession: NZ_CP073943
• Organism: Escherichia coli strain MB52

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7W4PV54
• Locus tag: KFU89_RS03515
• Protein ID: WP_000854821.1
• Coordinates: 718952..719329 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A4Q1HU91
• Locus tag: KFU89_RS03510
• Protein ID: WP_053287563.1
• Coordinates: 718494..718862 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU89_RS03480 (714461)	714461..715333	+	873	WP_053292456.1	GTPase family protein	-
KFU89_RS03485 (715502)	715502..715912	+	411	Protein_683	autotransporter adhesin family protein	-
KFU89_RS03490 (716239)	716239..717057	+	819	WP_053292454.1	DUF932 domain-containing protein	-
KFU89_RS03495 (717148)	717148..717633	+	486	WP_000214317.1	antirestriction protein	-
KFU89_RS03500 (717648)	717648..718124	+	477	WP_001186747.1	RadC family protein	-
KFU89_RS03505 (718193)	718193..718414	+	222	WP_000692309.1	DUF987 domain-containing protein	-
KFU89_RS03510 (718494)	718494..718862	+	369	WP_053287563.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
KFU89_RS03515 (718952)	718952..719329	+	378	WP_000854821.1	type IV toxin-antitoxin system cytoskeleton-binding toxin CbtA	Toxin
KFU89_RS03520 (719326)	719326..719523	+	198	Protein_690	DUF5983 family protein	-
KFU89_RS03525 (719551)	719551..719748	+	198	WP_088209831.1	DUF957 domain-containing protein	-
KFU89_RS03530 (719833)	719833..720678	+	846	WP_063101552.1	DUF4942 domain-containing protein	-
KFU89_RS03540 (720977)	720977..721483	+	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
KFU89_RS03545 (721562)	721562..723403	-	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	blaCTX-M-32	rfaE	693958..744242	50284

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13930.95 Da
• Isoelectric Point: 8.5163

>T200695 WP_000854821.1 NZ_CP073943:718952-719329 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T200695 NZ_CP098145:c1294424-1294206 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13663.36 Da
• Isoelectric Point: 6.4768

>AT200695 WP_053287563.1 NZ_CP073943:718494-718862 [Escherichia coli]
VSDTFSGTTHPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNSRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT200695 NZ_CP098145:c1294825-1294451 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• AlphaFold DB: A0A7W4PV54

Antitoxin

• AlphaFold DB: A0A4Q1HU91