Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataT-KacA/DUF1778(antitoxin)
Location 37152..37950 Replicon plasmid pYLMB53a
Accession NZ_CP073940
Organism Escherichia coli strain MB53

Toxin (Protein)


Gene name ataT Uniprot ID E2QDF3
Locus tag KFU83_RS22580 Protein ID WP_000072677.1
Coordinates 37429..37950 (+) Length 174 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID A0A0V9R6Q7
Locus tag KFU83_RS22575 Protein ID WP_001351987.1
Coordinates 37152..37421 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU83_RS22550 (KFU83_22375) 32579..33919 - 1341 WP_257188410.1 DnaB-like helicase C-terminal domain-containing protein -
KFU83_RS22555 (KFU83_22380) 33963..34703 - 741 WP_257188411.1 hypothetical protein -
KFU83_RS22560 (KFU83_22385) 34986..35753 + 768 WP_000342417.1 hypothetical protein -
KFU83_RS22565 (KFU83_22390) 35806..36159 - 354 WP_160378290.1 hypothetical protein -
KFU83_RS22570 (KFU83_22395) 36165..36833 - 669 WP_000161228.1 division plane positioning ATPase MipZ -
KFU83_RS22575 (KFU83_22400) 37152..37421 + 270 WP_001351987.1 DUF1778 domain-containing protein Antitoxin
KFU83_RS22580 (KFU83_22405) 37429..37950 + 522 WP_000072677.1 GNAT family N-acetyltransferase Toxin
KFU83_RS22585 (KFU83_22410) 38119..38370 - 252 WP_257188412.1 hypothetical protein -
KFU83_RS22590 (KFU83_22415) 38372..39064 - 693 WP_032084069.1 membrane protein -
KFU83_RS22595 (KFU83_22420) 39078..39401 - 324 WP_257188413.1 hypothetical protein -
KFU83_RS22600 (KFU83_22425) 39476..40264 - 789 WP_193131347.1 receptor-recognizing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..112732 112732


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(72-150)

Antitoxin

Vibrio phage ICP1, Orf50

(8-86)


Sequences

Toxin        


Download         Length: 174 a.a.        Molecular weight: 19392.17 Da        Isoelectric Point: 8.6369

>T200692 WP_000072677.1 NZ_CP073940:37429-37950 [Escherichia coli]
VSNTTIEIFSGEKDYDLNGFDCGEESLNAFLANHLKRQHEGKILRAYVLCTQEERPKVLGYYTLSGSCFEKESLPSRSKQ
KKVPYRNVPSVTLGRLALDKSLQGQGFGSMLVTHAMRVVYNASLAVGIHGLFVEALNDKAKAFYKSLGFIQLVGNNERSL
FYPTKSIEKLFEE

Download         Length: 522 bp

>T200692 NZ_CP098145:529443-529727 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGACCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10082.54 Da        Isoelectric Point: 7.0155

>AT200692 WP_001351987.1 NZ_CP073940:37152-37421 [Escherichia coli]
MSALKKQRIDLRLTDDDKSIIEEAAAMSNQSITQFMVSSASERAVKVIEQHRRLVLNEESWNLVMDAISNPPAPNDKLKR
AADRLKSME

Download         Length: 270 bp

>AT200692 NZ_CP098145:529211-529453 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGGTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0V9R6P3

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9R6Q7

References