Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3784588..3785138 Replicon chromosome
Accession NZ_CP073939
Organism Escherichia coli strain MB53

Toxin (Protein)


Gene name ccdB Uniprot ID Q32K46
Locus tag KFU83_RS18435 Protein ID WP_001160974.1
Coordinates 3784588..3784902 (-) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag KFU83_RS18440 Protein ID WP_000125566.1
Coordinates 3784905..3785138 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU83_RS18410 (3780263) 3780263..3781549 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
KFU83_RS18415 (3781549) 3781549..3782538 + 990 WP_062882910.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
KFU83_RS18420 (3782535) 3782535..3783356 + 822 WP_001065381.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
KFU83_RS18425 (3783359) 3783359..3783736 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
KFU83_RS18430 (3783743) 3783743..3784591 + 849 WP_000257184.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
KFU83_RS18435 (3784588) 3784588..3784902 - 315 WP_001160974.1 CcdB family protein Toxin
KFU83_RS18440 (3784905) 3784905..3785138 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
KFU83_RS18445 (3785224) 3785224..3785703 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
KFU83_RS18450 (3785895) 3785895..3787757 - 1863 WP_000377191.1 glutathione-regulated potassium-efflux system protein KefC -
KFU83_RS18455 (3787750) 3787750..3788280 - 531 WP_257188143.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
KFU83_RS18460 (3788391) 3788391..3789722 - 1332 WP_001183198.1 MFS transporter -
KFU83_RS18465 (3789781) 3789781..3790068 - 288 WP_000203741.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-103)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 11737.84 Da        Isoelectric Point: 9.8517

>T200685 WP_001160974.1 NZ_CP073939:c3784902-3784588 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFIRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T200685 NZ_CP098141:3891418-3891636 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT200685 WP_000125566.1 NZ_CP073939:c3785138-3784905 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT200685 NZ_CP098141:3891017-3891391 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LRR3

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References