Detailed information of TA system TA200682

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3409495..3410332
• Replicon: chromosome
• Accession: NZ_CP073939
• Organism: Escherichia coli strain MB53

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: KFU83_RS16550
• Protein ID: WP_000227784.1
• Coordinates: 3409790..3410332 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: KFU83_RS16545
• Protein ID: WP_001297137.1
• Coordinates: 3409495..3409806 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU83_RS16520 (3404515)	3404515..3405462	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
KFU83_RS16525 (3405484)	3405484..3407475	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
KFU83_RS16530 (3407465)	3407465..3408079	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
KFU83_RS16535 (3408079)	3408079..3408408	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
KFU83_RS16540 (3408420)	3408420..3409310	+	891	WP_000971336.1	heme o synthase	-
KFU83_RS16545 (3409495)	3409495..3409806	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
KFU83_RS16550 (3409790)	3409790..3410332	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
KFU83_RS16555 (3410388)	3410388..3411323	-	936	WP_001371638.1	tetratricopeptide repeat protein	-
KFU83_RS16560 (3411731)	3411731..3413095	+	1365	WP_001000954.1	MFS transporter	-
KFU83_RS16565 (3413223)	3413223..3413714	-	492	WP_040062457.1	nucleotide binding protein YajQ	-
KFU83_RS16570 (3413882)	3413882..3414793	+	912	WP_000705865.1	2-dehydropantoate 2-reductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T200682 WP_000227784.1 NZ_CP073939:3409790-3410332 [Escherichia coli]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T200682 NZ_CP098141:1433768-1434250 [Klebsiella pneumoniae]
GTGGGACGTGTAACCGCGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTTGA
TGAATGGCTAAAGCACAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGGACGA
AGCAAGTAGCTGGTTTTTACTCTCTGGCCACCGGTAGCGTTAACCATACGGAAACTACAGGCAGACTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCCGTTCTACGCTGTTATCGCGTAGCCGAGAATATTGGCGTCCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCTAAAGGCTTCTATACTCACCATGGATTTACGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT200682 WP_001297137.1 NZ_CP073939:3409495-3409806 [Escherichia coli]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT200682 NZ_CP098141:1433514-1433780 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCTTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3