Detailed information of TA system TA200680

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2388799..2389437
• Replicon: chromosome
• Accession: NZ_CP073939
• Organism: Escherichia coli strain MB53

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A2X7GI71
• Locus tag: KFU83_RS11620
• Protein ID: WP_032231106.1
• Coordinates: 2389261..2389437 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: KFU83_RS11615
• Protein ID: WP_001270286.1
• Coordinates: 2388799..2389215 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFU83_RS11595 (2384091)	2384091..2385299	+	1209	WP_001339197.1	IS4-like element ISVsa5 family transposase	-
KFU83_RS11600 (2385310)	2385310..2385906	-	597	Protein_2272	ABC transporter ATP-binding protein	-
KFU83_RS11605 (2385924)	2385924..2387069	-	1146	WP_000047429.1	ABC transporter substrate-binding protein	-
KFU83_RS11610 (2387314)	2387314..2388720	-	1407	WP_257188013.1	PLP-dependent aminotransferase family protein	-
KFU83_RS11615 (2388799)	2388799..2389215	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
KFU83_RS11620 (2389261)	2389261..2389437	-	177	WP_032231106.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
KFU83_RS11625 (2389659)	2389659..2389889	+	231	WP_000494244.1	YncJ family protein	-
KFU83_RS11630 (2389981)	2389981..2391942	-	1962	WP_001491066.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
KFU83_RS11635 (2392015)	2392015..2392551	-	537	WP_000429151.1	DNA-binding transcriptional regulator SutR	-
KFU83_RS11640 (2392643)	2392643..2393816	+	1174	Protein_2280	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2384091..2385299	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6680.72 Da
• Isoelectric Point: 10.9223

>T200680 WP_032231106.1 NZ_CP073939:c2389437-2389261 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPCHPSDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T200680 NZ_CP073939:c2389437-2389261 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGTGTCACCCTAGCGATGAGATTAAAGAACCATTGCGTAAAGCAATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT200680 WP_001270286.1 NZ_CP073939:c2389215-2388799 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT200680 NZ_CP073939:c2389215-2388799 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A2X7GI71