Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 268085..268716 Replicon chromosome
Accession NZ_CP073939
Organism Escherichia coli strain MB53

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag KFU83_RS01255 Protein ID WP_001259385.1
Coordinates 268085..268360 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag KFU83_RS01260 Protein ID WP_000593555.1
Coordinates 268357..268716 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFU83_RS01240 (263089) 263089..264156 + 1068 WP_040062220.1 HlyD family secretion protein -
KFU83_RS01245 (264153) 264153..266888 + 2736 WP_112046575.1 ribosome-associated ATPase/putative transporter RbbA -
KFU83_RS01250 (266888) 266888..268012 + 1125 WP_001314210.1 ABC transporter permease -
KFU83_RS01255 (268085) 268085..268360 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
KFU83_RS01260 (268357) 268357..268716 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
KFU83_RS01265 (268836) 268836..269237 - 402 WP_001190062.1 nickel-responsive transcriptional regulator NikR -
KFU83_RS01270 (269243) 269243..270049 - 807 WP_089601760.1 nickel import ATP-binding protein NikE -
KFU83_RS01275 (270046) 270046..270810 - 765 WP_089601759.1 nickel import ATP-binding protein NikD -
KFU83_RS01280 (270810) 270810..271643 - 834 WP_001008963.1 nickel ABC transporter permease subunit NikC -
KFU83_RS01285 (271640) 271640..272584 - 945 WP_000947068.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T200668 WP_001259385.1 NZ_CP073939:268085-268360 [Escherichia coli]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T200668 NZ_CP098136:c3134016-3133633 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT200668 WP_000593555.1 NZ_CP073939:268357-268716 [Escherichia coli]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT200668 NZ_CP098136:c3134258-3134016 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References